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A hydrogen-bonded electron-tunneling circuit reads the base composition of unmodified DNA

Jin He1, Lisha Lin1,2, Hao Liu1,2, Peiming Zhang1, Myeong Lee3, O F Sankey3 and S M Lindsay1,2,3

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Using a tunnel junction in which one electrode is guanidinium-functionalized (to trap DNA via hydrogen bonding to the backbone phosphates) and a second electrode which is functionalized with a base (to capture its complementary target on the DNA), current versus distance curves are obtained which yield an accurate measure of the base composition of DNA oligomers. With this long tunneling path, resolution is limited to sequence blocks of about twenty bases or larger, because of the need to form a large-area tunnel junction. A shorter hydrogen-bonded path across bases will be required for DNA sequencing. Nonetheless, these measurements point the way to a new type of nanoscale sensor.


PACS

87.15.B- Structure of biomolecules

87.15.N- Properties of solutions of macromolecules

87.15.R- Reactions and kinetics

07.07.Df Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing

87.80.-y Biophysical techniques (research methods)

87.14.G- Nucleic acids

Subjects

Instrumentation and measurement

Medical physics

Biological physics

Dates

Issue 7 (18 February 2009)

Received 13 October 2008, in final form 8 December 2008

Published 23 January 2009



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