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Multiplex detection of breast cancer biomarkers using plasmonic molecular sentinel nanoprobes

Hsin-Neng Wang1,2 and Tuan Vo-Dinh1,2,3,4

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We have demonstrated for the first time the feasibility of multiplex detection using the surface-enhanced Raman scattering-based molecular sentinel (MS) technology in a homogeneous solution. Two MS nanoprobes tagged with different Raman labels were used to detect the presence of the erbB-2 and ki-67 breast cancer biomarkers. The multiplexing capability of the MS technique was demonstrated by mixing the two MS nanoprobes and tested in the presence of single or multiple DNA targets.


PACS

87.85.Qr Nanotechnologies-design

87.14.G- Nucleic acids

87.19.X- Diseases

87.64.Cc Scattering of visible, uv, and infrared radiation

61.46.-w Structure of nanoscale materials

Subjects

Medical physics

Biological physics

Nanoscale science and low-D systems

Dates

Issue 6 (11 February 2009)

Received 28 September 2008, in final form 10 December 2008

Published 14 January 2009



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