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DNA origami as a nanoscale template for protein assembly

Anton Kuzyk1,2, Kimmo T Laitinen1 and Päivi Törmä2,3

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We describe two general approaches to the utilization of DNA origami structures for the assembly of materials. In one approach, DNA origami is used as a prefabricated template for subsequent assembly of materials. In the other, materials are assembled simultaneously with the DNA origami, i.e. the DNA origami technique is used to drive the assembly of materials. Fabrication of complex protein structures is demonstrated by these two approaches. The latter approach has the potential to be extended to the assembly of multiple materials with single attachment chemistry.


PACS

87.85.Qr Nanotechnologies-design

87.15.B- Structure of biomolecules

87.14.G- Nucleic acids

81.16.Dn Self-assembly

36.20.Hb Configuration (bonds, dimensions)

Subjects

Soft matter, liquids and polymers

Atomic and molecular physics

Biological physics

Nanoscale science and low-D systems

Dates

Issue 23 (10 June 2009)

Received 18 January 2009, in final form 30 March 2009

Published 18 May 2009



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