Shan Jiang et al 2009 Nanotechnology 20 155101 doi:10.1088/0957-4484/20/15/155101
Shan Jiang1, Yong Zhang1,2,6, Kian Meng Lim3, Eugene K W Sim4 and Lei Ye5
Show affiliationsNear-infrared (NIR)-to-visible upconversion fluorescent nanoparticles were synthesized and used for imaging and targeted delivery of small interfering RNA (siRNA) to cancer cells. Silica-coated NaYF4 upconversion nanoparticles (UCNs) co-doped with lanthanide ions (Yb/Er) were synthesized. Folic acid and anti-Her2 antibody conjugated UCNs were used to fluorescently label the folate receptors of HT-29 cells and Her2 receptors of SK-BR-3 cells, respectively. The intracellular uptake of the folic acid and antibody conjugated UCNs was visualized using a confocal fluorescence microscope equipped with an NIR laser. siRNA was attached to anti-Her2 antibody conjugated UCNs and the delivery of these nanoparticles to SK-BR-3 cells was studied. Meanwhile, a luciferase assay was established to confirm the gene silencing effect of siRNA. Upconversion nanoparticles can serve as a fluorescent probe and delivery system for simultaneous imaging and delivery of biological molecules.
Issue 15 (15 April 2009)
Received 15 December 2008
Published 24 March 2009
Shan Jiang et al 2009 Nanotechnology 20 155101
Sven Barth et al 2009 Nanotechnology 20 115705
C Q Peng et al 2008 Nanotechnology 19 505603
Keith A Brown and Robert M Westervelt 2009 Nanotechnology 20 385302
Kai Liu et al 2010 Nanotechnology 21 045708
Shamit Kachru et al JHEP03(2003)061
X Chen and K Jiang 2008 Nanotechnology 19 215305
Shamit Kachru et al JHEP10(2003)007
Hideo Kaiju et al 2010 Nanotechnology 21 015301
Jochen Weber et al 2008 Nanotechnology 19 375301