Eriko Mikamo-Satoh et al 2009 Nanotechnology 20 145102 doi:10.1088/0957-4484/20/14/145102
Electrostatic force microscopy: imaging DNA and protein polarizations one by one
Eriko Mikamo-Satoh1,2, Fumihiko Yamada1, Akihiko Takagi1, Takuya Matsumoto1,3 and Tomoji Kawai1,3
Show affiliationsWe present electrostatic force microscopy images of double-stranded DNA and transcription complex on an insulating mica substrate obtained with molecular resolution using a frequency-mode noncontact atomic force microscope. The electrostatic potential images show that both DNA and transcription complexes are polarized with an upward dipole moment. Potential differences of these molecules from the mica substrate enabled us to estimate dipole moments of isolated DNA and transcription complex in zero external field to be 0.027 D/base and 0.16 D/molecule, respectively. Scanning capacitance microscopy demonstrates characteristic contrast inversion between DNA and transcription complex images, indicating the difference in electric polarizability of these molecules. These findings indicate that the electrostatic properties of individual biological molecules can be imaged on an insulator substrate while retaining complex formation.
- PACS
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87.64.Dz Scanning tunneling and atomic force microscopy
87.15.R- Reactions and kinetics
- Subjects
- Dates
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Issue 14 (8 April 2009)
Received 24 October 2008, in final form 5 December 2008
Published 17 March 2009
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Electrostatic force microscopy: imaging DNA and protein polarizations one by one
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