Bifeng Pan et al 2009 Nanotechnology 20 125101 doi:10.1088/0957-4484/20/12/125101
Bifeng Pan1, Daxiang Cui1,6, Ping Xu1, Cengiz Ozkan2, Gao Feng1, Mihri Ozkan3, Tuo Huang1, Bingfeng Chu4, Qing Li1, Rong He1 and Guohan Hu5,6
Show affiliationsWith the aim of improving the amount and delivery efficiency of genes taken by carbon nanotubes into human cancer cells, different generations of polyamidoamine dendrimer modified multi-walled carbon nanotubes (dMNTs) were fabricated, and characterized by high-resolution transmission electron microscopy, atomic force microscopy, x-ray photoelectron spectroscopy, Raman spectroscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis, revealing the presence of dendrimer capped on the surface of carbon nanotubes. The dMNTs fully conjugated with FITC-labeled antisense c-myc oligonucleotides (asODN), those resultant asODN–dMNTs composites were incubated with human breast cancer cell line MCF-7 cells and MDA-MB-435 cells, and liver cancer cell line HepG2 cells, and confirmed to enter into tumor cells within 15 min by laser confocal microscopy. These composites inhibited the cell growth in time- and dose-dependent means, and down-regulated the expression of the c-myc gene and C-Myc protein. Compared with the composites of CNT–NH2–asODN and dendrimer–asODN, no. 5 generation of dendrimer-modified MNT–asODN composites exhibit maximal transfection efficiencies and inhibition effects on tumor cells. The intracellular gene transport and uptake via dMNTs should be generic for the mammalian cell lines. The dMNTs have potentials in applications such as gene or drug delivery for cancer therapy and molecular imaging.
81.16.-c Methods of nanofabrication and processing
78.30.Na Fullerenes and related materials
Condensed matter: electrical, magnetic and optical
Issue 12 (25 March 2009)
Received 23 May 2008, in final form 17 January 2009
Published 3 March 2009
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