P Mukherjee et al 2008 Nanotechnology 19 075103 doi:10.1088/0957-4484/19/7/075103
Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungus T. asperellum
P Mukherjee1, M Roy2, B P Mandal2, G K Dey3, P K Mukherjee1, J Ghatak4, A K Tyagi2 and S P Kale1,5
Show affiliationsA controlled and up-scalable biosynthetic route to nanocrystalline silver particles with well-defined morphology using cell-free aqueous filtrate of a non-pathogenic and commercially viable biocontrol agent Trichoderma asperellum is being reported for the first time. A transparent solution of the cell-free filtrate of Trichoderma asperellum containing 1 mM AgNO3 turns progressively dark brown within 5 d of incubation at 25 °C. The kinetics of the reaction was studied using UV–vis spectroscopy. An intense surface plasmon resonance band at ~410 nm in the UV–vis spectrum clearly reveals the formation of silver nanoparticles. The size of the silver particles using TEM and XRD studies is found to be in the range 13–18 nm. These nanoparticles are found to be highly stable and even after prolonged storage for over 6 months they do not show significant aggregation. A plausible mechanism behind the formation of silver nanoparticles and their stabilization via capping has been investigated using FTIR and surface-enhanced resonance Raman spectroscopy.
- PACS
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87.85.Qr Nanotechnologies-design
- Subjects
- Dates
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Issue 7 (20 February 2008)
Received 30 October 2007, in final form 14 December 2007
Published 29 January 2008
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Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungus T. asperellum
P Mukherjee et al 2008 Nanotechnology 19 075103
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