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High-speed AFM of human chromosomes in liquid

L M Picco1, P G Dunton1, A Ulcinas1, D J Engledew1, O Hoshi2, T Ushiki2 and M J Miles1

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Further developments of the previously reported high-speed contact-mode AFM are described. The technique is applied to the imaging of human chromosomes at video rate both in air and in water. These are the largest structures to have been imaged with high-speed AFM and the first imaging in liquid to be reported. A possible mechanism that allows such high-speed contact-mode imaging without significant damage to the sample is discussed in the context of the velocity dependence of the measured lateral force on the AFM tip.


PACS

87.64.Dz Scanning tunneling and atomic force microscopy

87.16.Sr Chromosomes, histones

87.15.B- Structure of biomolecules

Subjects

Medical physics

Biological physics

Dates

Issue 38 (24 September 2008)

Received 23 June 2008

Published 12 August 2008



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