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A new detection system for extremely small vertically mounted cantilevers

M Antognozzi1, A Ulcinas1, L Picco1, S H Simpson1, P J Heard2, M D Szczelkun3, B Brenner4 and M J Miles1

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Detection techniques currently used in scanning force microscopy impose limitations on the geometrical dimensions of the probes and, as a consequence, on their force sensitivity and temporal response. A new technique, based on scattered evanescent electromagnetic waves (SEW), is presented here that can detect the displacement of the extreme end of a vertically mounted cantilever. The resolution of this method is tested using different cantilever sizes and a theoretical model is developed to maximize the detection sensitivity. The applications presented here clearly show that the SEW detection system enables the use of force sensors with sub-micron size, opening new possibilities in the investigation of biomolecular systems and high speed imaging. Two types of cantilevers were successfully tested: a high force sensitivity lever with a spring constant of 0.17 pN nm−1 and a resonant frequency of 32 kHz; and a high speed lever with a spring constant of 50 pN nm−1 and a resonant frequency of 1.8 MHz. Both these force sensors were fabricated by modifying commercial microcantilevers in a focused ion beam system. It is important to emphasize that these modified cantilevers could not be detected by the conventional optical detection system used in commercial atomic force microscopes.


PACS

07.07.Df Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing

07.79.Lh Atomic force microscopes

07.10.Cm Micromechanical devices and systems

87.15.H- Dynamics of biomolecules

Subjects

Instrumentation and measurement

Biological physics

Dates

Issue 38 (24 September 2008)

Received 18 February 2008, in final form 17 March 2008

Published 12 August 2008



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