Dong Cai et al 2008 Nanotechnology 19 345102 doi:10.1088/0957-4484/19/34/345102
Interaction between carbon nanotubes and mammalian cells: characterization by flow cytometry and application
Dong Cai1,6, Derek Blair1, Fay J Dufort1, Maria R Gumina1, Zhongping Huang2, George Hong3, Dean Wagner4, D Canahan2, K Kempa5, Z F Ren5 and Thomas C Chiles1
Show affiliationsWe show herein that CNT–cell complexes are formed in the presence of a magnetic field. The complexes were analyzed by flow cytometry as a quantitative method for monitoring the physical interactions between CNTs and cells. We observed an increase in side scattering signals, where the amplitude was proportional to the amount of CNTs that are associated with cells. Even after the formation of CNT–cell complexes, cell viability was not significantly decreased. The association between CNTs and cells was strong enough to be used for manipulating the complexes and thereby conducting cell separation with magnetic force. In addition, the CNT–cell complexes were also utilized to facilitate electroporation. We observed a time constant from CNT–cell complexes but not from cells alone, indicating a high level of pore formation in cell membranes. Experimentally, we achieved the expression of enhanced green fluorescence protein by using a low electroporation voltage after the formation of CNT–cell complexes. These results suggest that higher transfection efficiency, lower electroporation voltage, and miniaturized setup dimension of electroporation may be accomplished through the CNT strategy outlined herein.
- PACS
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87.85.Qr Nanotechnologies-design
81.15.Gh Chemical vapor deposition (including plasma-enhanced CVD, MOCVD, etc.)
87.15.K- Molecular interactions; membrane-protein interactions
- Subjects
- Dates
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Issue 34 (27 August 2008)
Received 8 April 2008, in final form 10 June 2008
Published 15 July 2008
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Interaction between carbon nanotubes and mammalian cells: characterization by flow cytometry and application
Dong Cai et al 2008 Nanotechnology 19 345102
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-
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-
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-
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