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How nanoparticles encapsulating fluorophores allow a double detection of biomolecules by localized surface plasmon resonance and luminescence

G Barbillon1,2, A C Faure1, N El Kork1, P Moretti1, S Roux1, O Tillement1, M G Ou3, A Descamps3, P Perriat3,5, A Vial2, J-L Bijeon2, C A Marquette4 and B Jacquier1

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The paper shows how polysiloxane particles encapsulating fluorophores can be successfully used to detect biotin–streptavidin binding by two types of technique. After functionalization of the particles by streptavidin, the fixation of the biomolecule can indeed be detected by a shift of the localized surface plasmon resonance of the biotinylated gold dots used as substrate and by the luminescence of the fluorophores evidenced by scanning near-field optical microscopy. The development of particles allowing such a double detection opens a route for increasing the reliability of biological detection and for multi-labelling strategies crossing both detection principles.


PACS

87.85.Qr Nanotechnologies-design

87.64.mt Near-field scanning

87.80.-y Biophysical techniques (research methods)

87.15.M- Spectra of biomolecules

Subjects

Instrumentation and measurement

Medical physics

Biological physics

Dates

Issue 3 (23 January 2008)

Received 19 September 2007, in final form 29 October 2007

Published 11 December 2007



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