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Diffusion of nanoparticles into the capsule and cortex of a crystalline lens

Ronald A Schachar1,3, Wei Chen1, Boon K Woo1, Barbara K Pierscionek2, Xing Zhang1 and Lun Ma1

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The purpose of this study is to determine the ability of fluorescent nanoparticles to diffuse into a crystalline lens. Intact porcine lenses from five-month-old pigs, intact human lenses obtained from three donors aged 41, 42 and 45 years, and sections of human lens cortex obtained from four donors aged 11, 19, 32, and 34 years were incubated for 72 h at 7 °C in aqueous solutions of green (566 nm) and red (652 nm) fluorescent water soluble cadmium tellurium (CdTe) nanoparticles. As demonstrated by fluorescent and confocal microscopy, the CdTe nanoparticles diffused into the porcine and human lens capsule and into human cortical lens fibres; however, the nanoparticles did not pass through the intact lens capsule. Nanoparticles can be used as a method for studying intracellular structure and biochemical pathways within the lens capsule and cortical lens fibres to further understand cataractogenesis and may serve as a carrier for chemotherapeutic agents for the potential treatment of primary and secondary cataracts.


PACS

87.85.Qr Nanotechnologies-design

47.63.mh Transport processes and drug delivery

87.64.mk Confocal

42.66.Ct Anatomy and optics of eye

87.19.X- Diseases

Subjects

Fluid dynamics

Optics, quantum optics and lasers

Medical physics

Biological physics

Dates

Issue 2 (16 January 2008)

Received 17 August 2007, in final form 1 November 2007

Published 6 December 2007



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