Maryana Escalante et al 2008 Nanotechnology 19 025101 doi:10.1088/0957-4484/19/02/025101
Maryana Escalante1, Pascale Maury2, Christiaan M Bruinink2, Kees van der Werf1, John D Olsen3, John A Timney3, Jurriaan Huskens2, C Neil Hunter3, Vinod Subramaniam1,4 and Cees Otto1,4
Show affiliationsWe report the directed assembly of the photosynthetic membrane proteins LH1 and LH2 isolated from the purple bacterium Rhodobacter sphaeroides onto chemically patterned substrates. Nanoimprint lithography was used to pattern discrete regions of amino- and fluoro-terminated or poly(ethylene glycol) self-assembled monolayers onto a glass substrate. Densely packed layers of assembled protein complexes were observed with atomic force microscopy. The protein complexes attached selectively to the amino-terminated regions by electrostatic interactions. Spectral images generated with a hybrid scanning probe and fluorescence microscope confirmed that the patterned proteins retained their native optical signatures.
87.85.Qr Nanotechnologies-design
87.15.M- Spectra of biomolecules
Issue 2 (16 January 2008)
Received 23 May 2007, in final form 18 October 2007
Published 6 December 2007
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