Quick search Find article
Quick search
Find article

Large-area protein nano-arrays patterned by soft lithography

A Ruiz, A Valsesia, F Bretagnol, P Colpo and F Rossi

Show affiliations


Nanostructured polydimethylsiloxane stamps have been used to pattern regular nano-arrays of proteins on polyethylene glycol surfaces by microcontact printing. The master was fabricated by using colloidal lithography. In this technique polystyrene nanobeads were used as nanomasks during subsequent oxygen etching and silica deposition. The elastomeric stamps, fabricated by classical replica moulding, reproduced accurately the nanosized features of the moulds. Printing of the proteins was performed by controlling the pressure, enabling the creation of an ordered arrangement of fouling domains (protein) on a non-fouling background (polyethylene glycol). 300 nm diameter poly-L-lysine spots spaced 100 nm were patterned. Such nanofabricated biofunctional surfaces offer promising capabilities for the development of highly compacted, ultra-sensitive biosensing devices.


PACS

87.85.Qr Nanotechnologies-design

81.16.Rf Nanoscale pattern formation

81.16.Fg Supramolecular and biochemical assembly

81.16.Nd Nanolithography

87.14.E- Proteins

87.15.B- Structure of biomolecules

Subjects

Biological physics

Nanoscale science and low-D systems

Dates

Issue 50 (19 December 2007)

Received 20 August 2007, in final form 5 October 2007

Published 23 November 2007



View by subject




Export








Please login to access our web services, or create an account if you don't yet have one.

You must have cookies enabled in your web browser to be able to login.

Username
Password

Forgotten your password? Get a new one here.