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Analysis of structural order in amyloid fibrils

Tuomas P J Knowles1,2, Jeffrey F Smith1,2, Glyn L Devlin3, Christopher M Dobson2,3 and Mark E Welland1

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We discuss the application of atomic force microscopy to characterize supra-molecular ordering in elongated protein aggregates such as amyloid fibrils. The measurements reveal that the one-dimensional defect density along these structures is comparable to that found in true three-dimensional macromolecular crystals. The high level of structural order has important implications for understanding the nature of the interactions responsible for protein aggregation, and sheds light on the involvement of amyloid fibrils in aberrant biological pathways.


PACS

87.15.B- Structure of biomolecules

87.15.K- Molecular interactions; membrane-protein interactions

87.14.E- Proteins

87.15.N- Properties of solutions of macromolecules

Subjects

Biological physics

Dates

Issue 4 (31 January 2007)

Received 25 September 2006, in final form 6 November 2006

Published 21 December 2006



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