Xiaoxiao He et al 2007 Nanotechnology 18 365604 doi:10.1088/0957-4484/18/36/365604
Xiaoxiao He, Yingjie Chen, Kemin Wang1, Ping Wu, Ping Gong and Hailing Huo
Show affiliationsTwo kinds of unique pH-dependent magnetic nanoadsorbents based on silica coated magnetic nanoparticles (SMNPs) and amino-silica coated magnetic nanoparticles (ASMNPs) have been exploited for selective separation of proteins. With different isoelectric points, silica coated magnetic nanoadsorbents (SMNAs) and amino-silica coated magnetic nanoadsorbents (ASMNAs) can respectively adsorb proteins with different charges. The interactions between proteins and magnetic nanoadsorbents changed with the solution pH. Thus, the adsorption or desorption between proteins and magnetic nanoadsorbents can be controlled by changing the solution pH according to the charge of the proteins. And the magnetic nanoadsorbents can be separated and recycled simply with a magnet. As model adsorbates, Cyt-c and BSA are selectively separated from simple protein mixtures with SMNAs and ASMNAs respectively. Cyt-c was adsorbed on SMNAs at pH 8.0 at 0.160 mg mg−1. And BSA was adsorbed on ASMNAs at pH 5.0 at 0.142 mg mg−1.
75.50.Tt Fine-particle systems; nanocrystalline materials
Issue 36 (12 September 2007)
Received 10 May 2007, in final form 18 July 2007
Published 10 August 2007
Xiaoxiao He et al 2007 Nanotechnology 18 365604
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