Kuang-Kai Liu et al 2007 Nanotechnology 18 325102 doi:10.1088/0957-4484/18/32/325102
Kuang-Kai Liu1,2, Chia-Liang Cheng3, Chia-Ching Chang4,5,6 and Jui-I Chao1,2,7
Show affiliationsSurface-modified carboxylated nanometre-sized diamond (cND) has been applied for the conjugation of biological molecules such as DNA and protein. In this study, we evaluated the biocompatibility and detection of cNDs and carbon nanotubes on human lung A549 epithelial cells and HFL-1 normal fibroblasts. Treatment with 5 or 100 nm cND particles, 0.1–100 µg ml−1, did not reduce the cell viability and alter the protein expression profile in lung cells; however, carbon nanotubes induced cytotoxicity in these cells. The cNDs particles were accumulated in A549 cells, which were observed by atomic force microscopy and laser scanning confocal microscopy. Both 5 and 100 nm cNDs particles exhibited the green fluorescence and were ingested into cells. Moreover, the fluorescence intensities were increased in cells via a concentration-dependent manner after treatment with 5 and 100 nm cNDs, which can be detected by flow cytometer analysis. The fluorescence intensities of 5 nm cNDs were relative higher than 100 nm cNDs in cells at equal concentration treatment. The observation demonstrated that cND-interacting with cell is detectable by a confocal microscope, flow cytometer and atomic force microscope. These nanoparticles may be useful for further biomedical applications based on the properties of uptake ability, detectability and little cytotoxicity in human cells.
87.85.Qr Nanotechnologies-design
Issue 32 (15 August 2007)
Received 24 May 2007
Published 13 July 2007
Kuang-Kai Liu et al 2007 Nanotechnology 18 325102
G Helesfai 2008 Class. Quantum Grav. 25 235010
Mark J Kushner 2009 J. Phys. D: Appl. Phys. 42 194013
J Erö et al 2008 JINST 3 P08006
Pradipta Kumar Mandal and Debnarayan Jana 2009 J. Phys. A: Math. Theor. 42 485004
N M Souza-Neto et al 2009 J. Phys.: Conf. Ser. 190 012112
P Murugavel et al 2008 J. Phys.: Condens. Matter 20 415208
Rong Xiang et al 2007 Nanotechnology 18 415703
G Belenky et al 2009 Semicond. Sci. Technol. 24 115013
J Stötzel et al 2009 J. Phys.: Conf. Ser. 190 012162