Elena Perevedentseva et al 2007 Nanotechnology 18 315102 doi:10.1088/0957-4484/18/31/315102
Elena Perevedentseva1, Chih-Yuan Cheng, Pei-Hua Chung, Jhih-Sian Tu, Yu-Hsin Hsieh and Chia-Liang Cheng2
Show affiliationsThe application of a nanometre-sized diamond in Raman-detectable biolabelling is demonstrated in this study. The interaction of a lysozyme–nanodiamond complex with bacteria E. coli was observed via Raman mapping using the diamond Raman signal as the labelling marker. The results are compared with scanning electron microscope observations, and the adsorbed lysozyme's functionality is analysed. High antibacterial activity of lysozyme–nanodiamond complex was observed, equivalent to active lysozyme in solution. The results suggest that nanodiamond labelling can be effective and that it can be applied in ambient conditions without complicated sample pre-treatments.
87.85.Qr Nanotechnologies-design
87.15.K- Molecular interactions; membrane-protein interactions
Issue 31 (8 August 2007)
Received 22 February 2007, in final form 13 April 2007
Published 6 July 2007
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