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Trapping of 27 bp–8 kbp DNA and immobilization of thiol-modified DNA using dielectrophoresis

Sampo Tuukkanen1, Anton Kuzyk1, J Jussi Toppari1, Hannu Häkkinen2, Vesa P Hytönen3,4, Einari Niskanen3, Marcus Rinkiö1 and Päivi Törmä1

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Dielectrophoretic trapping of six different DNA fragments, sizes varying from 27 to 8416 bp, has been studied using confocal microscopy. The effect of the DNA length and the size of the constriction between nanoscale fingertip electrodes on the trapping efficiency have been investigated. Using finite element method simulations in conjunction with the analysis of the experimental data, the polarizabilities of the different size DNA fragments have been calculated for different frequencies. Also the immobilization of trapped hexanethiol- and DTPA-modified 140 nm long DNA to the end of gold nanoelectrodes was experimentally quantified and the observations were supported by density functional theory calculations.


PACS

87.85.Qr Nanotechnologies-design

87.15.B- Structure of biomolecules

87.14.G- Nucleic acids

87.64.mk Confocal

87.15.Tt Electrophoresis

87.15.A- Theory, modeling, and computer simulation

Subjects

Medical physics

Biological physics

Dates

Issue 29 (25 July 2007)

Received 3 May 2007, in final form 30 May 2007

Published 20 June 2007



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