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Characterization of enhanced antibacterial effects of novel silver nanoparticles

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Siddhartha Shrivastava1,5, Tanmay Bera2,5, Arnab Roy1, Gajendra Singh3, P Ramachandrarao4 and Debabrata Dash1,6

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In the present study, we report the preparation of silver nanoparticles in the range of 10–15 nm with increased stability and enhanced anti-bacterial potency. The morphology of the nanoparticles was characterized by transmission electron microscopy. The antibacterial effect of silver nanoparticles used in this study was found to be far more potent than that described in the earlier reports. This effect was dose dependent and was more pronounced against gram-negative bacteria than gram-positive organisms. Although bacterial cell lysis could be one of the reasons for the observed antibacterial property, nanoparticles also modulated the phosphotyrosine profile of putative bacterial peptides, which could thus affect bacterial signal transduction and inhibit the growth of the organisms.


PACS

87.85.Qr Nanotechnologies-design

87.17.Ee Growth and division

68.37.Lp Transmission electron microscopy (TEM)

87.16.Xa Signal transduction and intracellular signaling

61.46.Df Structure of nanocrystals and nanoparticles ("colloidal" quantum dots but not gate-isolated embedded quantum dots)

Subjects

Surfaces, interfaces and thin films

Biological physics

Nanoscale science and low-D systems

Dates

Issue 22 (6 June 2007)

Received 19 January 2007, in final form 14 February 2007

Published 4 May 2007



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