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Electron flux through apo-and holoferritin

Danny N Axford and Jason J Davis

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Conductive probe atomic force microscopy (CP-AFM) has been used to investigate electronic transport through the protein ferritin in both its holo and apo forms. The presence of the iron oxide core has a notable effect on both conductance and the molecular response to probe-induced compression. This response can also be contrasted with that of the much smaller metalloprotein cytochrome c, across which electron transport can be simulated by a single non-resonant tunnel barrier model. Tapping mode AFM imaging, in different compressional regimes, reveals both the mineral core of holoferritin and significant collapse of the hollow protein cavity of apoferritin. These topographic findings correlate well with CP-AFM conductance data and facilitate a clearer description of electron transport across these molecules.


PACS

87.14.E- Proteins

87.64.Dz Scanning tunneling and atomic force microscopy

82.39.Jn Charge (electron, proton) transfer in biological systems

87.85.Qr Nanotechnologies-design

87.16.Uv Active transport processes

Subjects

Medical physics

Biological physics

Chemical physics and physical chemistry

Dates

Issue 14 (11 April 2007)

Received 1 December 2006, in final form 29 January 2007

Published 6 March 2007



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