Ingrid Hilger et al 2007 Nanotechnology 18 135103 doi:10.1088/0957-4484/18/13/135103
Ingrid Hilger1,5, René Trost1, Jürgen R Reichenbach2,5, Werner Linß3, Marcus-René Lisy1, Alexander Berndt4 and Werner A Kaiser1,2
Show affiliationsThe aim of this study was to assess whether Her-2/neu expressing tumour cells can be detected in vitro as well as in animal tumour models with magnetic resonance imaging at 1.5 T. Magnetic nanoparticles (with relaxivities R 1, R 2 of 3.7 ± 0.4 (mM s)−1, 277 ± 32 (mM s)−1 at 21 °C, respectively) coupled to anti-Her-2/neu antibodies or gamma globulin IgG (high or non-affinity probe, respectively) were used. After incubation of Her-2/neu expressing cells (SKBR3) with high or non-affinity probes (20 min), values of R 1 = 0.34 ± 0.02 (mM s)−1 and R 2 = 63.02 ± 30 (mM s)−1 were obtained. Electron microscopy and atomic absorption spectrometry examinations verified the presence of relatively high iron levels in cells incubated with the high affinity probe compared to controls. For in vivo MRI, high or non-affinity probes (≈1.7 mg Fe/animal) were injected into the tail vein of mice (n = 16) bearing SKBR3 tumours. A distinct decrease in the normalized MR signal ratio between tumour and reference area (approximately −17 ± 2%) after application of the high affinity probe was observed. In conclusion, in vivo detection of Her-2/neu expressing tumours is feasible in a clinical MR scanner by using immunoconjugated magnetic nanoparticles.
87.85.Qr Nanotechnologies-design
75.50.Tt Fine-particle systems; nanocrystalline materials
Issue 13 (4 April 2007)
Received 22 August 2006, in final form 8 January 2007
Published 28 February 2007
Ingrid Hilger et al 2007 Nanotechnology 18 135103
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