Quick search Find article
Quick search
Find article

Imaging of biomaterials in liquids: a comparison between conventional and Q-controlled amplitude modulation ('tapping mode') atomic force microscopy

D Ebeling1,2, H Hölscher1,2, H Fuchs1,2, B Anczykowski3 and U D Schwarz4

Show affiliations


Lambda phage DNA and DPPC thin films are imaged in liquids by atomic force microscopy applying the amplitude modulation mode ('tapping mode') with active enhancement of the Q-factor by a 'Q-control' electronics. The topography of the resulting images is compared with images obtained without active Q-control. To enable a meaningful comparison, individual scan lines are alternately recorded with and without Q-factor enhancement using scan parameters optimized for each mode separately. As the major finding, significant height differences of topographical features are observed between the two modes. The heights measured with active Q-control are reproducibly higher compared to the ones observed without Q enhancement. This effect is attributed to the reduction of tip–sample forces by Q-control.


PACS

87.64.Dz Scanning tunneling and atomic force microscopy

68.37.Ps Atomic force microscopy (AFM)

87.14.G- Nucleic acids

Subjects

Surfaces, interfaces and thin films

Medical physics

Biological physics

Dates

Issue 7 (14 April 2006)

Received 22 August 2005, in final form 4 December 2005

Published 10 March 2006



  1. Imaging of biomaterials in liquids: a comparison between conventional and Q-controlled amplitude modulation ('tapping mode') atomic force microscopy

    D Ebeling et al 2006 Nanotechnology 17 S221

  2. β-decay of 22O

    L Weissman et al 2005 J. Phys. G: Nucl. Part. Phys. 31 553

  3. Interpretation of the atomic scale contrast obtained on graphite and single-walled carbon nanotubes in the dynamic mode of atomic force microscopy

    Makoto Ashino et al 2005 Nanotechnology 16 S134

  4. Muon acceleration with a very fast ramping synchrotron for a neutrino factory

    D J Summers et al 2003 J. Phys. G: Nucl. Part. Phys. 29 1727

  5. Ionization cooling in FFAGs: progress since NuFact'01

    H Schönauer 2003 J. Phys. G: Nucl. Part. Phys. 29 1739

  6. Exploitation of spectral direct UV irradiance measurements

    Raul R Cordero et al 2009 Metrologia 46 19

  7. The neutrino superbeam from the AGS

    W T Weng et al 2003 J. Phys. G: Nucl. Part. Phys. 29 1735

  8. The Boomerang Nebula: The Coldest Region of the Universe?

    Raghvendra Sahai and Lars-Åke Nyman 1997 ApJ 487 L155

  9. A giant natural TPC (500 km)3 to observe extremely high energy cosmic particles - JEM EUSO telescope on International Space Station

    Y Takahashi 2007 J. Phys.: Conf. Ser. 65 012022

  10. Simulation of strong magnetospheric disturbances in laser-produced plasma experiments

    A G Ponomarenko et al 2008 Plasma Phys. Control. Fusion 50 074015

View by subject




Export








Please login to access our web services, or create an account if you don't yet have one.

You must have cookies enabled in your web browser to be able to login.

Username
Password

Forgotten your password? Get a new one here.