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Self-aligned immobilization of proteins utilizing PEG patterns

Lap Man Lee1, Ronald L Heimark2, James C Baygents3 and Yitshak Zohar1

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A novel self-aligned method to selectively immobilize proteins on a silicon dioxide surface is developed in conjunction with a standard lift-off patterning technique of a PEG layer. The approach is designed to photolithographically pattern regions that specifically bind target proteins and particles, surrounded by regions that suppress non-specific attachment of bio-species. The physical and biological properties of the derivatized surfaces at the end of the fabrication process are characterized.


PACS

87.15.B- Structure of biomolecules

81.16.Nd Nanolithography

87.14.E- Proteins

87.64.Dz Scanning tunneling and atomic force microscopy

81.16.Dn Self-assembly

Subjects

Medical physics

Biological physics

Nanoscale science and low-D systems

Dates

Issue 4 (28 February 2006)

Received 26 July 2005

Published 25 January 2006



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