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Improved metal cluster deposition on a genetically engineered tobacco mosaic virus template

Sang-Yup Lee1, Elizabeth Royston1, James N Culver2 and Michael T Harris1,3

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Improved depositions of various metal clusters onto a biomolecular template were achieved using a genetically engineered tobacco mosaic virus (TMV). Wild-type TMV was genetically altered to display multiple solid metal binding sites through the insertion of two cysteine residues within the amino-terminus of the virus coat protein. Gold, silver, and palladium clusters synthesized through in situ chemical reductions could be readily deposited onto the genetically modified template via the exposed cysteine-derived thiol groups. Metal cluster coatings on the cysteine-modified template were more densely deposited and stable than similar coatings on the unmodified wild-type template. Combined, these results confirm that the introduction of cysteine residues onto the outer surface of the TMV coat protein enhances the usefulness of this virus as a biotemplate for the deposition of metal clusters.


PACS

87.85.Lf Tissue engineering

87.14.E- Proteins

87.15.B- Structure of biomolecules

87.85.Qr Nanotechnologies-design

87.16.Nn Motor proteins (myosin, kinesin dynein)

61.46.-w Structure of nanoscale materials

Subjects

Biological physics

Nanoscale science and low-D systems

Dates

Issue 7 (July 2005)

Received 8 January 2005, in final form 13 April 2005

Published 3 May 2005



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