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Rapid functionalization of cantilever array sensors by inkjet printing

Alexander Bietsch1,2,3, Jiayun Zhang1, Martin Hegner1, Hans Peter Lang1,2 and Christoph Gerber1,2

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The controlled deposition of functional layers is the key to converting nanomechanical cantilevers into chemical or biochemical sensors. Here, we introduce inkjet printing as a rapid and general method to coat cantilever arrays efficiently with various sensor layers. Self-assembled monolayers of alkanethiols were deposited on selected Au-coated cantilevers and rendered them sensitive to ion concentrations or pH in liquids. The detection of gene fragments was achieved with cantilever sensors coated with thiol-linked single-stranded DNA oligomers on Au. A selective etch protocol proved the uniformity of the monolayer coatings at a microscopic level. A chemical gas sensor was fabricated by printing thin layers of different polymers from dilute solutions onto cantilevers. The inkjet method is easy to use, faster and more versatile than coating via microcapillaries or the use of pipettes. In addition, it is scalable to large arrays and can coat arbitrary structures in non-contact.


PACS

87.80.-y Biophysical techniques (research methods)

81.16.Dn Self-assembly

68.55.-a Thin film structure and morphology

87.14.G- Nucleic acids

Subjects

Instrumentation and measurement

Surfaces, interfaces and thin films

Medical physics

Biological physics

Nanoscale science and low-D systems

Dates

Issue 8 (August 2004)

Received 8 April 2004

Published 9 June 2004



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