J S Bunch et al 2004 Nanotechnology 15 S76 doi:10.1088/0957-4484/15/2/016
J S Bunch1, T N Rhodin2 and P L McEuen1
Show affiliationsSingle-molecule imaging at nanometre resolution using noncontact AFM has been applied to image a protein complex on mica using single-wall carbon nanotube (CNT) technology. Hydrated RNA polymerase (RNAP)–DNA complex was imaged using constant-amplitude tapping mode AFM. CNT tips (1–2 nm diameter) prepared by chemical vapour phase deposition were mounted onto standard Pt/Ir coated AFM tips. The effectiveness of the CNT tip scanning was tested by imaging the profile of an RNAP–DNA complex. The results demonstrate the merits of high-resolution carbon nanotube nc-AFM scanning and suggest that CNT tips may provide a general tip–surface reference standard for defining tip–surface interactions on molecular-bonded organic surfaces.
81.16.Fg Supramolecular and biochemical assembly
68.47.Pe Langmuir-Blodgett films on solids; polymers on surfaces; biological molecules on surfaces
Issue 2 (February 2004)
Received 22 September 2003
Published 22 January 2004
J S Bunch et al 2004 Nanotechnology 15 S76
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