V Baranauskas et al 2004 Nanotechnology 15 1661 doi:10.1088/0957-4484/15/11/050
Analysis of the coagulation of human blood cells on diamond surfaces by atomic force microscopy
V Baranauskas, M Fontana, Zhao Jing Guo, H J Ceragioli and A C Peterlevitz
Show affiliationsAtomic force microscopy (AFM) was used to study the morphology and coagulation of human blood cells in contact with solid surfaces. Blood was extracted from the veins of healthy adult donors and the samples were used immediately after extraction, deposited either on borosilicate glass or diamond substrates. Some blood samples were anti-coagulated by adding heparin for single cell AFM imaging. No chemicals were used for attaching or immobilizing the cells. The diamond substrates were produced by chemical vapour deposition (CVD diamond) using a hot-filament CVD system fed with ethanol highly diluted in hydrogen. AFM imaging of isolated cells (anti-coagulated by heparin) was only possible on the glass substrates due to the lack of adherence of the cells to the diamond surface. The coagulation results suggest that blood clotting on diamond produces a less rough surface than blood clotting on glass.
- PACS
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87.64.Dz Scanning tunneling and atomic force microscopy
68.47.Pe Langmuir-Blodgett films on solids; polymers on surfaces; biological molecules on surfaces
83.80.Lz Physiological materials (e.g. blood, collagen, etc.)
- Subjects
- Dates
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Issue 11 (November 2004)
Received 12 July 2004, in final form 24 September 2004
Published 22 October 2004
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Analysis of the coagulation of human blood cells on diamond surfaces by atomic force microscopy
V Baranauskas et al 2004 Nanotechnology 15 1661
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