Absar Ahmad et al 2003 Nanotechnology 14 824 doi:10.1088/0957-4484/14/7/323
Absar Ahmad1,5, Satyajyoti Senapati2, M Islam Khan1, Rajiv Kumar2, R Ramani3, V Srinivas3 and Murali Sastry4,5
Show affiliationsThe development of reliable, eco-friendly processes for the synthesis of nanoscale materials is an important aspect of nanotechnology. In this paper, we report on the use of an alkalotolerant actinomycete (Rhodococcus sp.) in the intracellular synthesis of gold nanoparticles of the dimension 5–15 nm. Electron microscopy analysis of thin sections of the gold actinomycete cells indicated that gold particles with good monodispersity were formed on the cell wall as well as on the cytospasmic membrane. The particles are more concentrated on the cytoplasmic membrane than on the cell wall, possibly due to reduction of the metal ions by enzymes present in the cell wall and on the cytoplasmic membrane. The metal ions were not toxic to the cells and the cells continued to multiply after biosynthesis of the gold nanoparticles.
87.16.-b Subcellular structure and processes
87.85.Qr Nanotechnologies-design
81.16.Fg Supramolecular and biochemical assembly
81.07.Bc Nanocrystalline materials
Issue 7 (July 2003)
Received 22 April 2003
Published 6 June 2003
Absar Ahmad et al 2003 Nanotechnology 14 824
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