Quick search Find article
Quick search
Find article

Observation of microtubules with scanning force microscopy in liquid

P J de Pablo1, I A T Schaap and C F Schmidt

Show affiliations


We present the application of scanning force microscopy using the jumping mode to investigate microtubules adsorbed to glass in air and in liquid. To fix the microtubules the glass surfaces were silanized with aminopropyl-triethoxy-silane. The observed structures ranged from disrupted microtubules in air to intact microtubules in liquid. Intact microtubules show heights between 20 and 24 nm confirming the diameter found in electron microscopy studies. The force applied by the tip was critical for the microtubule height, indicating deformation by the tip. Internal structure, corresponding to protofilaments, was found.


PACS

68.37.Ps Atomic force microscopy (AFM)

68.43.Mn Adsorption kinetics

Subjects

Surfaces, interfaces and thin films

Dates

Issue 2 (February 2003)

Received 16 September 2002

Published 10 January 2003



  1. Observation of microtubules with scanning force microscopy in liquid

    P J de Pablo et al 2003 Nanotechnology 14 143

  2. Density of water: roles of the CIPM and IAPWS standards

    A H Harvey et al 2009 Metrologia 46 196

  3. On the Tolman and Møller mass-energy formulae in general relativity

    P S Florides 2009 J. Phys.: Conf. Ser. 189 012014

  4. Solitary wave solution for a non-integrable, variable coefficient nonlinear Schrödinger equation

    R Grimshaw et al 2007 Phys. Scr. 75 620

  5. Comments on photonic shells

    M Žofka 2004 Class. Quantum Grav. 21 465

  6. Quantum interference injection and control of spin-polarized transient current gratings in GaAs

    H M van Driel et al 2004 Semicond. Sci. Technol. 19 S223

  7. Acoustic properties of some liquid Tl-Te alloys

    R Turner 1974 J. Phys. C: Solid State Phys. 7 3686

  8. Excitation of axisymmetric Alfvénic modes in Ohmic tokamak discharges

    K.G. McClements et al 2002 Nucl. Fusion 42 1155

  9. Changes to the VNIIM air kerma primary standard

    I A Kharitonov et al 2007 Metrologia 44 L71

  10. Photonic crystal light-emitting diodes fabricated by microsphere lithography

    W N Ng et al 2008 Nanotechnology 19 255302

Related review articles

What's this?
View review articles related to this research to gain an insight into the key trends in this subject area. Related review articles are selected based on PACS/MSC codes, and are no more than three years old.

  1. Processes involving clusters and small particles in a buffer gas
  2. Oxide nanostructures on a Nb surface and related systems: experiments and ab initio calculations
  3. Atomic resolution non-contact atomic force microscopy of clean metal oxide surfaces

View by subject




Export








Please login to access our web services, or create an account if you don't yet have one.

You must have cookies enabled in your web browser to be able to login.

Username
Password

Forgotten your password? Get a new one here.