Andrea Csáki et al 2003 Nanotechnology 14 1262 doi:10.1088/0957-4484/14/12/006
Andrea Csáki, Pia Kaplanek, Robert Möller and Wolfgang Fritzsche
Show affiliationsThe detection of DNA using nanoparticles as labels is an interesting alternative to the standard fluorescence technique. It requires simpler detection equipment, resulting in higher stability and lower costs. Besides easier detection, metal enhancement allows a higher sensitivity of detection. The signal-response curve for labelled DNA before and after silver enhancement was studied, applying both atomic force microscope (AFM) and optical (reflection/transmission) measurements. The dynamic range and the sensitivity were determined for nanoparticle labelling with and without metal enhancement. Nanoparticle concentrations down to the fM range could be detected. The ultimate limit of detection, the identification of individual labels, is demonstrated for the optical readout. Therefore, AFM images of the particles were correlated with the optical signal of individual or clustered particles. We demonstrate that the optical signal allows the identification of single particles.
Issue 12 (December 2003)
Received 30 June 2003, in final form 13 October 2003
Published 22 October 2003
Andrea Csáki et al 2003 Nanotechnology 14 1262
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