Y Arntz et al 2003 Nanotechnology 14 86 doi:10.1088/0957-4484/14/1/319
Y Arntz1, J D Seelig1, H P Lang1,2, J Zhang1, P Hunziker1, J P Ramseyer1, E Meyer1, M Hegner1 and Ch Gerber1,2
Show affiliationsWe demonstrate continuous label-free detection of two cardiac biomarker proteins (creatin kinase and myoglobin) using an array of microfabricated cantilevers functionalized with covalently anchored anti-creatin kinase and anti-myoglobin antibodies. This method allows biomarker proteins to be detected via measurement of surface stress generated by antigen–antibody molecular recognition. Reference cantilevers are used to eliminate thermal drifts, undesired chemical reactions and turbulences from injections of liquids by calculating differential deflection signals with respect to sensor cantilevers. The sensitivity achieved for myoglobin detection is below 20 µg ml−1. Both myoglobin and creatin kinase could be detected independently using cantilevers functionalized with the corresponding antibodies, in unspecific protein background. This approach permits the use of up to seven different antigen–antibody reactions simultaneously, including an additional thermomechanical and chemical in situ reference. Applications lie in the field of early and rapid diagnosis of acute myocardial infarction.
87.15.K- Molecular interactions; membrane-protein interactions
Issue 1 (January 2003)
Received 31 October 2002
Published 20 December 2002
Y Arntz et al 2003 Nanotechnology 14 86
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R F Sabirianov et al 2007 J. Phys.: Condens. Matter 19 082201
Ramin M Abolfath et al 2007 New J. Phys. 9 353
Gonzalo Alvarez et al J. Stat. Mech. (2007) P08007
Miguel Fuentes-Cabrera et al 2007 Nanotechnology 18 424019
Y F Guan et al 2007 Nanotechnology 18 335306
Tae-Hwan Kim et al 2008 Nanotechnology 19 485201
Meng-Dawn Cheng et al 2007 Nanotechnology 18 185604
J D Fowlkes et al 2008 Nanotechnology 19 415301