Andrew F Dias et al 2002 Nanotechnology 13 285 doi:10.1088/0957-4484/13/3/309
Andrew F Dias, Gregor Dernick, Vicente Valero, Ming G Yong, Conrad D James, Harold G Craighead and Manfred Lindau1
Show affiliationsNanobiotechnology is a field that utilizes the techniques of nano- and microfabrication to study biosystems or to use biological material and principles to build new devices. As an example we discuss the development of a nanofabricated electrochemical detector array that reveals the spatio-temporal dynamics of exocytosis in single chromaffin cells. In a quantal release event a single vesicle fuses with the plasma membrane releasing its contents through the fusion pore. The time-resolved amperometric currents measured by the individual electrodes detecting different fractions of the released molecules allow determination of the time course as well as localization of quantal events. Such a device may be applicable to study the correlation of exocytotic events with signalling events that could be simultaneously monitored by fluorescence microscopy.
87.85.Qr Nanotechnologies-design
Issue 3 (June 2002)
Received 3 December 2001, in final form 30 April 2002
Published 23 May 2002
Andrew F Dias et al 2002 Nanotechnology 13 285
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