Ocular fluorometry is a non-invasive diagnostic technique that has been used widely in research to measure the amount of fluorescein leakage from the blood into the ocular tissues and fluids after intravenous injection. This information has been demonstrated to be valuable in a number of clinically relevant situations. This paper mainly deals with demonstrating how the use of modern sensors associated with effective temperature control can configure a low-cost solution to an ocular fluorometry instrument and still upgrade the performance previously obtained with bench-top units only. A new ocular fluorometer is briefly described and special attention is dedicated to the temperature cooling and control system that has been developed and to the quantification of its effects on the detector signal-to-noise ratio (SNR), lowest level of detection (LLOD) and error of measurement (EOM) within the context of ocular fluorometry requirements. Experimental evidence is given that shows not only the improvements in LLOD with moderate cooling (more then 35% for a decrease in temperature in the range), but also the attenuation of the EOM by temperature stabilization ( uncertainty in the range induces a EOM), the current LLOD being lower then , as we will see.