Based on the operational concept of quasi-static state and optoelectronic measurement technology, this research develops a real-time dynamic imaging system for centrifugal microfluidic platforms. Unlike the conventional centrifugal inspection system, which can only be used for examination of the final steady stage in microflow analysis, the developed system with a multi-speed controller and object tracking design is fabricated with low cost to recognize dynamic microflow patterns for different kinds of compact disc-type centrifugal microstructures. The characteristics of rotational control efficiency and image acquisition quality are obtained from fluidic microvalve experiments that are achieved in measuring microflow dynamic responses and in visualizing transient microflow patterns. A man–machine interface was connected with a computer to perform the control and alignment adjustments to catch exact image data for following analysis. The rotation stability of the system has been evaluated, and the rotation speed up to 4500 rpm with vertical vibration less than ±0.2 mm is achieved measured at radial distance of 5 cm. The image acquisition is transferred via USB 2.0 at a speed of up to 30 images per second to the display and memory module.