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Recent trends in electrochemical DNA biosensor technology

REVIEW ARTICLE

Kagan Kerman, Masaaki Kobayashi and Eiichi Tamiya1

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REVIEW ARTICLE

Recent trends and challenges in the electrochemical methods for the detection of DNA hybridization are reviewed. Electrochemistry has superior properties over the other existing measurement systems, because electrochemical biosensors can provide rapid, simple and low-cost on-field detection. Electrochemical measurement protocols are also suitable for mass fabrication of miniaturized devices. Electrochemical detection of hybridization is mainly based on the differences in the electrochemical behaviour of the labels towards the hybridization reaction on the electrode surface or in the solution. Basic criteria for electrochemical DNA biosensor technology, and already commercialized products, are also introduced. Future prospects towards PCR-free DNA chips are discussed.


PACS

87.80.-y Biophysical techniques (research methods)

82.45.Tv Bioelectrochemistry

87.14.G- Nucleic acids

82.47.Rs Electrochemical sensors

Subjects

Instrumentation and measurement

Medical physics

Biological physics

Chemical physics and physical chemistry

Dates

Issue 2 (February 2004)

Received 2 October 2003, accepted for publication 7 November 2003

Published 11 December 2003



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