Masao Kamahori et al 2002 Meas. Sci. Technol. 13 1779 doi:10.1088/0957-0233/13/11/314
Masao Kamahori, Kunio Harada and Hideki Kambara
Show affiliationsEasy and inexpensive single nucleotide polymorphisms (SNPs) typing systems are required for the practice of genetic testing as well as genetic medicine. Most of the SNPs typing systems use laser-induced fluorescence detection coupled with fluorophore tagging on DNA, which are expensive. A new simple and inexpensive SNPs typing system is presented. It uses a bioluminometric assay coupled with modified primer extension reactions and an inexpensive photodiode array for the luminometric detection. The reagents consumed in the assay are also inexpensive. Although the system is very small, simple and inexpensive, it gives enough sensitivity for detecting target DNAs as small as 10 fmol, which is good enough for SNPs typing.
87.15.M- Spectra of biomolecules
87.50.wj Dosimetry/exposure assessment
85.60.Dw Photodiodes; phototransistors; photoresistors
Issue 11 (November 2002)
Received 3 April 2002, accepted for publication 16 May 2002, in final form 15 May 2002
Published 3 October 2002
Masao Kamahori et al 2002 Meas. Sci. Technol. 13 1779
Uriel Giveon et al. 2002 ApJ 566 880
R Acharya and P Narayana Swamy 2004 J. Phys. A: Math. Gen. 37 6605
W. F. Welsh et al. 1998 ApJ 509 118
Salvatore Antoci et al 2001 Class. Quantum Grav. 18 3463
David A Meyer 2001 J. Phys. A: Math. Gen. 34 6981
Radoslaw Szmytkowski and Jürgen Hinze 1996 J. Phys. A: Math. Gen. 29 6125
C. Peralta and A. Melatos 2009 ApJ 701 L75
S S Manna and A Kabakçioglu 2003 J. Phys. A: Math. Gen. 36 L279
Jianfeng Feng et al 2003 J. Phys. A: Math. Gen. 36 7469