T Kaindl et al 2010 J. Phys.: Condens. Matter 22 285102 doi:10.1088/0953-8984/22/28/285102
T Kaindl1, J Oelke1,2, A Pasc3, S Kaufmann1, O V Konovalov4, S S Funari5, U Engel6, A Wixforth2 and M Tanaka1
Show affiliationsHighly uniform, strongly correlated domains of synthetically designed lipids can be incorporated into supported lipid membranes. The systematic characterization of membranes displaying a variety of domains revealed that the equilibrium size of domains significantly depends on the length of fluorocarbon chains, which can be quantitatively interpreted within the framework of an equivalent dipole model. A mono-dispersive, narrow size distribution of the domains enables us to treat the inter-domain correlations as two-dimensional colloidal crystallization and calculate the potentials of mean force. The obtained results demonstrated that both size and inter-domain correlation can precisely be controlled by the molecular structures. By coupling α-D-mannose to lipid head groups, we studied the adhesion behavior of the murine macrophage (J774A.1) on supported membranes. Specific adhesion and spreading of macrophages showed a clear dependence on the density of functional lipids. The obtained results suggest that such synthetic lipid domains can be used as a defined platform to study how cells sense the size and distribution of functional molecules during adhesion and spreading.
87.16.D- Membranes, bilayers, and vesicles
Issue 28 (21 July 2010)
Received 27 October 2009, in final form 11 March 2010
Published 15 June 2010
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