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Growth–melt asymmetry in ice crystals under the influence of spruce budworm antifreeze protein

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Natalya Pertaya1, Yeliz Celik1, Carlos L DiPrinzio1, J S Wettlaufer2,3, Peter L Davies4 and Ido Braslavsky1,5

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Thumbnail, link to movie 1
Movie 1.
(2.5 MB MP4) Bright field microscopy movie of the growth and melt of an ice crystal in sbwAFP solution, within a solution droplet suspended in oil in the nanoliter-osmometer apparatus. It shows the 30° rotation of the growing shapes compared to the melting shapes.

Thumbnail, link to movie 2
Movie 2.
(667 kB MP4) Fluorescence microscopy movie of growth and melt of an ice crystal in sbwAFP-GFP solution, within a two coverslip thin cell. It shows where the antifreeze proteins accumulate during ice growth and melting.



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