B Polak et al 2007 J. Phys.: Condens. Matter 19 285216 doi:10.1088/0953-8984/19/28/285216
B Polak1, R Olkowski2, T Kobiela3,4, M Lewandowska-Szumieł2 and W Fabianowski1
Show affiliationsSi supports for cell culture were modified using poly(acrylic acid) (PAA) and bentonite in order to obtain 'sandwich'-like structures. A layer of PAA cast from water solution was followed with a bentonite layer also cast from water dispersion, then another PAA layer and so on up to six layers. The prepared surfaces had different physical and chemical properties like thickness, topography and elasticity. Chemical composition was characterized by Raman spectroscopy. The elastic properties and topography of modified sandwich-like surfaces were evaluated using nanoindentation and atomic force microscopy measurements. In the next step bone cells were cultured on such modified surfaces composed of one to six layers. The influence of the substrate surface properties on the growth and behaviour of human bone derived cells (HBDC) was studied. The influence of surface topography, elasticity and chemical composition on cells is discussed.
68.35.B- Structure of clean surfaces (and surface reconstruction)
78.68.+m Optical properties of surfaces
81.40.Jj Elasticity and anelasticity, stress-strain relations
Soft matter, liquids and polymers
Condensed matter: electrical, magnetic and optical
Issue 28 (18 July 2007)
Received 30 September 2006, in final form 13 March 2007
Published 25 June 2007
B Polak et al 2007 J. Phys.: Condens. Matter 19 285216
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