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Structural rigidity in the capsid assembly of cowpea chlorotic mottle virus

B M Hespenheide1, D J Jacobs2 and M F Thorpe1

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The cowpea chlorotic mottle virus (CCMV) has a protein cage, or capsid, which encloses its genetic material. The structure of the capsid consists of 180 copies of a single protein that self-assemble inside a cell to form a complete capsid with icosahedral symmetry. The icosahedral surface can be naturally divided into pentagonal and hexagonal faces, and the formation of either of these faces has been proposed to be the first step in the capsid assembly process. We have used the software FIRST to analyse the rigidity of pentameric and hexameric substructures of the complete capsid to explore the viability of certain capsid assembly pathways. FIRST uses the 3D pebble game to determine structural rigidity, and a brief description of this algorithm, as applied to body–bar networks, is given here. We find that the pentameric substructure, which corresponds to a pentagonal face on the icosahedral surface, provides the best structural properties for nucleating the capsid assembly process, consistent with experimental observations.


PACS

87.14.E- Proteins

02.50.Le Decision theory and game theory

87.17.Aa Modeling, computer simulation of cell processes

87.15.B- Structure of biomolecules

87.16.-b Subcellular structure and processes

87.15.A- Theory, modeling, and computer simulation

MSC

91A80 Applications of game theory

92C37 Cell biology

92E10 Molecular structure (graph-theoretic methods, methods of differential topology, etc.)

Subjects

Computational physics

Biological physics

Dates

Issue 44 (10 November 2004)

Received 31 August 2004

Published 22 October 2004



  1. Structural rigidity in the capsid assembly of cowpea chlorotic mottle virus

    B M Hespenheide et al 2004 J. Phys.: Condens. Matter 16 S5055

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