T Koch 2003 J. Phys.: Condens. Matter 15 S1861 doi:10.1088/0953-8984/15/18/317
T Koch
Show affiliationsThe structure and synthesis of LNA (locked nucleic acid) is presented. LNA is defined as an oligonucleotide containing one or more 2'-O, 4'-C-methylene-β-D-ribofuranosyl nucleotide monomer(s). The improved synthesis of the thymine LNA monomer is shown. The LNA molecular family is presented and the member classes are described in relation to their chemical structures. The hybridization property of each member is shown and similarities and differences in the properties of the members are discussed. Hybridization kinetics of oxy-LNA oligonucleotides is shown and it is concluded that the hybridization rate is nearly diffusion controlled. Finally, the perspective of the broad biological application of LNA is discussed.
87.15.B- Structure of biomolecules
Issue 18 (14 May 2003)
Received 14 November 2002
Published 28 April 2003
T Koch 2003 J. Phys.: Condens. Matter 15 S1861
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