C. B. Müller et al 2008 EPL 83 46001 doi:10.1209/0295-5075/83/46001
C. B. Müller1, A. Loman2, V. Pacheco3, F. Koberling4, D. Willbold3, W. Richtering1 and J. Enderlein2
Show affiliationsDual-focus fluorescence correlation spectroscopy is a method for precisely measuring the diffusion coefficient of fluorescing molecules close to the infinite dilution limit in a reference-free and absolute manner. We apply the method to determine the diffusion coefficients of three fluorescent dyes across the visible spectrum. These values can be used as absolute reference standards for fluorescence correlation spectroscopy. In particular, it is found that the diffusion coefficient of the widely used reference dye Rhodamine 6G is by 37% larger than the value used in most publications on fluorescence correlation spectroscopy over the last three decades.
Issue 4 (August 2008)
Received 14 April 2008, accepted for publication 25 June 2008
Published 4 August 2008
C. B. Müller et al 2008 EPL 83 46001
E. Figuerêdo et al. 2008 The Astronomical Journal 136 221