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Salt-induced condensation in actin-DNA mixtures

O. V. Zribi1, H. Kyung1, R. Golestanian2,3, T. B. Liverpool2,4 and G. C. L. Wong1

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Multivalent ions can induce condensation of like-charged polyelectrolytes into compact states, a process that requires different ion valence for different polyelectrolyte species. We have examined the trivalent ion-induced condensation behavior in binary anionic polyelectrolyte mixtures consisting of DNA coils and F-actin rods, and observe a micro-phase separation between the two polyelectrolytes into coexisting finite-sized F-actin bundles and DNA toroids. Further, by increasing the DNA volume fraction in the mixture, condensed F-actin bundles can be completely destabilized, leading to only DNA condensation within the mixture. We examine a number of possible causes and propose a model based on polyelectrolyte competition for ions.


PACS

82.35.Pq Biopolymers, biopolymerization

87.15.Aa Theory and modeling; computer simulation

82.35.Rs Polyelectrolytes

Subjects

Soft matter, liquids and polymers

Biological physics

Chemical physics and physical chemistry

Dates

Issue 4 (May 2005)

Received 19 October 2004, accepted for publication 24 March 2005, in final form 24 March 2005

Published 22 April 2005



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