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Confocal optical microscopy

REVIEW ARTICLE

Robert H Webb

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Confocal optical microscopy is a technique for increasing the contrast of microscope images, particularly in thick specimens. By restricting the observed volume, the technique keeps overlying or nearby scatterers from contributing to the detected signal. The price for this is that the instrument must observe only one point at a time (in the scanning laser version) or a group of separated points with very little light (in the disc version). This paper describes how the confocal advantage comes about and how it is implemented in actual instruments.

This review was received in October 1995


PACS

07.60.Pb Conventional optical microscopes

42.30.Lr Modulation and optical transfer functions

42.79.Bh Lenses, prisms and mirrors

07.79.-v Scanning probe microscopes and components

42.79.Ls Scanners, image intensifiers, and image converters

42.79.Fm Reflectors, beam splitters, and deflectors

Subjects

Instrumentation and measurement

Optics, quantum optics and lasers

Dates

Issue 3 (March 1996)



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