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Audio frequency in vivo optical coherence elastography

Steven G Adie1, Brendan F Kennedy, Julian J Armstrong, Sergey A Alexandrov and David D Sampson

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We present a new approach to optical coherence elastography (OCE), which probes the local elastic properties of tissue by using optical coherence tomography to measure the effect of an applied stimulus in the audio frequency range. We describe the approach, based on analysis of the Bessel frequency spectrum of the interferometric signal detected from scatterers undergoing periodic motion in response to an applied stimulus. We present quantitative results of sub-micron excitation at 820 Hz in a layered phantom and the first such measurements in human skin in vivo.


 

General scientific summary. We present a novel optical coherence elastography technique to investigate the elastic properties of tissue in the hundred hertz to kilohertz range. The technique uses optical coherence tomography, which is based on low-coherence interferometry, to detect the vibration amplitude of tissue introduced by an actuator. We describe extraction of the vibration amplitude based on analysis of the Bessel frequency spectrum of the detected interferometric signal. We present results using 820 Hz excitation that clearly distinguish between the layers in a three-layer phantom. The first measurements of the elastic properties of human skin in vivo based on optical coherence tomography, also at 820 Hz, are presented. It is estimated that the Young's modulus of the epidermis is 3.7±0.2 times greater than that of the dermis. This technique could provide a new high-resolution contrast mechanism to aid physicians in the detection of various tissue pathologies, including cancer.

PACS

87.63.L- Visual imaging

07.60.Ly Interferometers

42.30.Wb Image reconstruction; tomography

87.19.R- Mechanical and electrical properties of tissues and organs

Subjects

Instrumentation and measurement

Biological physics

Optics, quantum optics and lasers

Medical physics

Dates

Issue 10 (21 May 2009)

Received 1 December 2008, in final form 3 April 2009

Published 6 May 2009



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