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A functionalized THz sensor for marker-free DNA analysis

M Nagel, F Richter, P Haring-Bolívar and H Kurz

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We report on a novel resonant THz sensor for the label-free analysis of DNA molecules. The sensor allows the direct detection of DNA-probe molecules at functionalized electrodes via hybridization. Subsequent time resolved photoconductive sampling of the THz transmission identifies the binding state between probe and target DNA. Integrating neighbouring sensors on a chip, this technique can be extended to a parallel analysis of multiple DNA sequences. A clearly readable sensor response is obtained with less then 40 fmol of 20-mer single-stranded DNA molecules, indicating at least a sevenfold sensitivity increase compared to previous approaches.


PACS

87.80.-y Biophysical techniques (research methods)

07.57.-c Infrared, submillimeter wave, microwave and radiowave instruments and equipment

87.15.Cc Folding: thermodynamics, statistical mechanics, models, and pathways

87.15.B- Structure of biomolecules

87.15.H- Dynamics of biomolecules

87.14.G- Nucleic acids

Subjects

Instrumentation and measurement

Medical physics

Biological physics

Dates

Issue 22 (21 November 2003)

Received 2 June 2003

Published 24 October 2003



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