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Far-infrared vibrational modes of DNA components studied by terahertz time-domain spectroscopy

B M Fischer, M Walther and P Uhd Jepsen

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The far-infrared dielectric function of a wide range of organic molecules is dominated by vibrations involving a substantial fraction of the atoms forming the molecule and motion associated with intermolecular hydrogen bond vibrations. Due to their collective nature such modes are highly sensitive to the intra- and intermolecular structure and thus provide a unique fingerprint of the conformational state of the molecule and effects of its environment. We demonstrate the use of terahertz time-domain spectroscopy (THz-TDS) for recording the far-infrared (0.5–4.0 THz) dielectric function of the four nucleobases and corresponding nucleosides forming the building blocks of deoxyribose nucleic acid (DNA). We observe numerous distinct spectral features with large differences between the molecules in both frequency-dependent absorption coefficient and index of refraction. Assisted by results from density-functional calculations we interpret the origin of the observed resonances as vibrations of hydrogen bonds between the molecules.


PACS

87.64.K- Spectroscopy

87.15.B- Structure of biomolecules

87.15.H- Dynamics of biomolecules

87.19.R- Mechanical and electrical properties of tissues and organs

07.57.Pt Submillimeter wave, microwave and radiowave spectrometers; magnetic resonance spectrometers, auxiliary equipment, and techniques

87.14.G- Nucleic acids

Subjects

Instrumentation and measurement

Medical physics

Biological physics

Dates

Issue 21 (7 November 2002)

Received 1 March 2002

Published 17 October 2002



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