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Melanin and blood concentration in human skin studied by multiple regression analysis: experiments

M Shimada1, Y Yamada2,3, M Itoh4 and T Yatagai4

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Knowledge of the mechanism of human skin colour and measurement of melanin and blood concentration in human skin are needed in the medical and cosmetic fields. The absorbance spectrum from reflectance at the visible wavelength of human skin increases under several conditions such as a sunburn or scalding. The change of the absorbance spectrum from reflectance including the scattering effect does not correspond to the molar absorption spectrum of melanin and blood. The modified Beer–Lambert law is applied to the change in the absorbance spectrum from reflectance of human skin as the change in melanin and blood is assumed to be small. The concentration of melanin and blood was estimated from the absorbance spectrum reflectance of human skin using multiple regression analysis. Estimated concentrations were compared with the measured one in a phantom experiment and this method was applied to in vivo skin.


PACS

87.64.Cc Scattering of visible, uv, and infrared radiation

87.80.-y Biophysical techniques (research methods)

82.80.Yc Rutherford backscattering (RBS), and other methods of chemical analysis

02.70.Rr General statistical methods

Subjects

Computational physics

Instrumentation and measurement

Medical physics

Biological physics

Chemical physics and physical chemistry

Dates

Issue 9 (September 2001)

Received 27 April 2001

Published 22 August 2001



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