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Atomic force microscope cantilever calibration device for quantified force metrology at micro- or nano-scale regime: the nano force calibrator (NFC)

Min-Seok Kim1, Jae-Hyuk Choi, Yon-Kyu Park and Jong-Ho Kim

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Motivated by emerging needs for accurate force measurements in the nanotechnology and biophysics areas, we present an atomic force microscope (AFM) cantilever calibration system, the 'nano force calibrator' (NFC), consisting of a microbalance and a precision translation stage. Calibration using the NFC has proved to be a reliable and accurate method through a series of experiments with a commercial piezoresistive AFM cantilever. In these experiments, linearity, repeatability and reproducibility of measurements were investigated along with the effects of calibration conditions, such as orientation of the cantilever and temperature. Uncertainty analysis shows that the stiffness and force sensitivity are determined to be 3.385 N m−1 and 0.6490 µN Ω−1, which are traceable to the Système International d'Unités (SI units). The relative standard uncertainties of both the stiffness and sensitivity are approximately 0.4% or conservatively 0.5%.


PACS

07.79.Lh Atomic force microscopes

07.10.Lw Balance systems, tensile machines, etc.

06.20.fb Standards and calibration

Subjects

Instrumentation and measurement

Dates

Issue 5 (October 2006)

Received 8 February 2006

Published 6 September 2006



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